Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211.

ABSTRACT: Salinomycin is widely used in animal husbandry as a food additive due to its antibacterial and anticoccidial activities. However, its biosynthesis had only been studied by feeding experiments with isotope-labeled precursors. A strategy with degenerate primers based on the polyether-specific epoxidase sequences was successfully developed to clone the salinomycin gene cluster. Using this strategy, a putative epoxidase gene, slnC, was cloned from the salinomycin producer Streptomyces albus XM211. The targeted replacement of slnC and subsequent trans-complementation proved its involvement in salinomycin biosynthesis. A 127-kb DNA region containing slnC was sequenced, including genes for polyketide assembly and release, oxidative cyclization, modification, export, and regulation. In order to ga

SUBMITTER: Jiang C 

PROVIDER: S-EPMC3272990 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature