Gene expression profiles of gliomas in formalin-fixed paraffin-embedded material.

ABSTRACT:

Background

We have recently demonstrated that expression profiling is a more accurate and objective method to classify gliomas than histology. Similar to most expression profiling studies, our experiments were performed using fresh frozen (FF) glioma samples whereas most archival samples are fixed in formalin and embedded in paraffin (FFPE). Identification of the same, expression-based intrinsic subtypes in FFPE-stored samples would enable validation of the prognostic value of these subtypes on these archival samples. In this study, we have therefore determined whether the intrinsic subtypes identified using FF material can be reproduced in FFPE-stored samples.

Methods

We have performed expression profiling on 55 paired FF-FFPE glioma samples using HU133 plus 2.0 arrays (FF)

SUBMITTER: Gravendeel LA 

PROVIDER: S-EPMC3273349 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature