Sequence composition and gene content of the short arm of rye (Secale cereale) chromosome 1.

ABSTRACT:

Background

The purpose of the study is to elucidate the sequence composition of the short arm of rye chromosome 1 (Secale cereale) with special focus on its gene content, because this portion of the rye genome is an integrated part of several hundreds of bread wheat varieties worldwide.

Methodology/principal findings

Multiple Displacement Amplification of 1RS DNA, obtained from flow sorted 1RS chromosomes, using 1RS ditelosomic wheat-rye addition line, and subsequent Roche 454FLX sequencing of this DNA yielded 195,313,589 bp sequence information. This quantity of sequence information resulted in 0.43× sequence coverage of the 1RS chromosome arm, permitting the identification of genes with estimated probability of 95%. A detailed analysis revealed that more than 5% of the 1RS

SUBMITTER: Fluch S 

PROVIDER: S-EPMC3273464 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature