Oral immunization of the African clawed frog (Xenopus laevis) upregulates the mucosal immunoglobulin IgX.

ABSTRACT: The frog Xenopus laevis is a model species for developmental biology but is also of significant interest to comparative immunologists. Amphibians are the oldest group of organisms in which both the B lymphocytes of some species undergo immunoglobulin (Ig) class switch recombination and also have a dedicated mucosal Ig isotype. The purpose of this study was to test the hypothesis that frog IgX would be produced in response to oral immunization. In order to facilitate studies of humoral, and especially mucosal immunity, in this model species, we developed a gavage technique for oral immunization. The result of this oral administration of antigen to frogs was assayed by the induction of the mucosal antibody isotype, IgX, in plasma by enzyme linked immunosorbant assay (ELISA), and a significan

SUBMITTER: Du CC 

PROVIDER: S-EPMC3273591 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature