Systematic screening for novel, serologically reactive Hepatitis E Virus epitopes.

ABSTRACT:

Background

The National Institutes of Health classified Hepatitis E as an emerging disease since Hepatitis E Virus (HEV) is the major cause of acute hepatitis in developing countries. Interestingly, an increasing number of sporadic cases of HEV infections are described in industrialized countries as zoonosis from domestic livestock. Despite the increasing relevance of this pathogen in clinical virology, commercial antibody assays are mainly based on fragments of HEV open reading frame (ORF) 2 and ORF3. The largest ORF1 (poly-)protein, however, is not part of current testing formats.

Methods

From a synthesized full length HEV genotype 1 cDNA-bank we constructed a complete HEV gene library consisting of 15 respective HEV ORF domains. After bacterial expression and purification

SUBMITTER: Osterman A 

PROVIDER: S-EPMC3274478 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature