Transcriptome analysis of the model protozoan, Tetrahymena thermophila, using Deep RNA sequencing.

ABSTRACT:

Background

The ciliated protozoan Tetrahymena thermophila is a well-studied single-celled eukaryote model organism for cellular and molecular biology. However, the lack of extensive T. thermophila cDNA libraries or a large expressed sequence tag (EST) database limited the quality of the original genome annotation.

Methodology/principal findings

This RNA-seq study describes the first deep sequencing analysis of the T. thermophila transcriptome during the three major stages of the life cycle: growth, starvation and conjugation. Uniquely mapped reads covered more than 96% of the 24,725 predicted gene models in the somatic genome. More than 1,000 new transcribed regions were identified. The great dynamic range of RNA-seq allowed detection of a nearly six order-of-magnitude range

SUBMITTER: Xiong J 

PROVIDER: S-EPMC3274533 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature