Comparative analysis of nuclear transfer embryo-derived mouse embryonic stem cells. Part I: cellular characterization.

ABSTRACT: Embryonic stem cells derived from nuclear transfer embryos (ntESCs) are particularly valuable for regenerative medicine, as they are a patient-specific and histocompatible cell source for the treatment of varying diseases. However, currently, little is known about their cellular and molecular profile. In the present study, in a mouse model different donor cell-derived ntESCs from various genetic backgrounds were compared with reference ESCs and analyzed comprehensively at the cellular level. A number of pluripotency marker genes were compared by flow cytometry and immunocytochemistry analysis. Significant differences at the protein level were observed for POU5F1, SOX2, FGF4, NANOG, and SSEA-1. However, such differences had no effect on in vitro cell differentiation and cell fate: derivativ

SUBMITTER: Kobolak J 

PROVIDER: S-EPMC3275094 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature