Human fetal liver stromal cells expressing erythropoietin promote hematopoietic development from human embryonic stem cells.

ABSTRACT: Blood cells transfusion and hematopoietic stem cells (HSCs) transplantation are important methods for cell therapy. They are widely used in the treatment of incurable hematological disorder, infectious diseases, genetic diseases, and immunologic deficiency. However, their availability is limited by quantity, capacity of proliferation and the risk of blood transfusion complications. Recently, human embryonic stem cells (hESCs) have been shown to be an alternative resource for the generation of hematopoietic cells. In the current study, we describe a novel method for the efficient production of hematopoietic cells from hESCs. The stable human fetal liver stromal cell lines (hFLSCs) expressing erythropoietin (EPO) were established using the lentiviral system. We observed that the supernatant

SUBMITTER: Yang C 

PROVIDER: S-EPMC3275106 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature