Discovery and verification of gelsolin as a potential biomarker of colorectal adenocarcinoma in the Chinese population: Examining differential protein expression using an iTRAQ labelling-based proteomics approach.

ABSTRACT:

Objective

To identify and validate potential biomarkers of colorectal adenocarcinoma using a proteomic approach.

Methods

Multidimensional liquid chromatography⁄mass spectrometry was used to analyze biological samples labelled with isobaric mass tags for relative and absolute quantitation to identify differentially expressed proteins in human colorectal adenocarcinoma and paired normal mucosa for the discovery of cancerous biomarkers. Cancerous and noncancerous samples were compared using online and offline separation. Protein identification was performed using mass spectrometry. The downregulation of gelsolin protein in colorectal adenocarcinoma samples was confirmed by Western blot analysis and validated using immunohistochemistry.

Results

A total of 802 nonredundant

SUBMITTER: Fan NJ 

PROVIDER: S-EPMC3275404 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature