Transforming growth factor-beta increases the expression of vascular smooth muscle cell markers in human multi-lineage progenitor cells.

ABSTRACT:

Background

Vascular smooth muscle cell (SMC) differentiation is an essential component of vascular repair and tissue engineering. However, currently used cell models for the study of SMC differentiation have several limitations. Multi-lineage progenitor cells (MLPCs) originate from human umbilical cord blood and are cloned from a single cell. The object of this study was to investigate whether MLPCs could differentiate into SMCs in vitro with induction by transforming growth factor beta1 (TGF-beta1).

Material/methods

MLPCs were treated without or with TGF-beta1 (1 and 5 ng/mL) in mesenchymal stem cell media plus 1% FBS for 7 days. Total RNA was isolated from the MLPCs, and semi-quantitative real-time PCR was performed to test the following mRNA levels: early and late phase S

SUBMITTER: Yang H 

PROVIDER: S-EPMC3276078 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature