A male sterility-associated cytotoxic protein ORF288 in Brassica juncea causes aborted pollen development.

ABSTRACT: Cytoplasmic male sterility (CMS) is a widespread phenomenon in higher plants, and several studies have established that this maternally inherited defect is often associated with a mitochondrial mutant. Approximately 10 chimeric genes have been identified as being associated with corresponding CMS systems in the family Brassicaceae, but there is little direct evidence that these genes cause male sterility. In this study, a novel chimeric gene (named orf288) was found to be located downstream of the atp6 gene and co-transcribed with this gene in the hau CMS sterile line. Western blotting analysis showed that this predicted open reading frame (ORF) was translated in the mitochondria of male-sterile plants. Furthermore, the growth of Escherichia coli was significantly repressed in the presence

SUBMITTER: Jing B 

PROVIDER: S-EPMC3276091 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature