Genome-Wide Analysis of Nascent Transcription in Saccharomyces cerevisiae.

ABSTRACT: The assessment of transcriptional regulation requires a genome-wide survey of active RNA polymerases. Thus, we combined the nuclear run-on assay, which labels and captures nascent transcripts, with high-throughput DNA sequencing to examine transcriptional activity in exponentially growing Saccharomyces cerevisiae. Sequence read data from these nuclear run-on libraries revealed that transcriptional regulation in yeast occurs not only at the level of RNA polymerase recruitment to promoters but also at postrecruitment steps. Nascent synthesis signals are strongly enriched at TSS throughout the yeast genome, particularly at histone loci. Nascent transcripts reveal antisense transcription for more than 300 genes, with the read data providing support for the activity of distinct promoters drivin

SUBMITTER: McKinlay A 

PROVIDER: S-EPMC3276176 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature