Identification of substrain-specific mutations by massively parallel whole-genome resequencing of Synechocystis sp. PCC 6803.

ABSTRACT: The cyanobacterium, Synechocystis sp. PCC 6803, was the first photosynthetic organism whose genome sequence was determined in 1996 (Kazusa strain). It thus plays an important role in basic research on the mechanism, evolution, and molecular genetics of the photosynthetic machinery. There are many substrains or laboratory strains derived from the original Berkeley strain including glucose-tolerant (GT) strains. To establish reliable genomic sequence data of this cyanobacterium, we performed resequencing of the genomes of three substrains (GT-I, PCC-P, and PCC-N) and compared the data obtained with those of the original Kazusa strain stored in the public database. We found that each substrain has sequence differences some of which are likely to reflect specific mutations that may contribute

SUBMITTER: Kanesaki Y 

PROVIDER: S-EPMC3276265 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature