Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses.

ABSTRACT: More than 120 human papillomaviruses (HPVs) have now been identified and have been associated with a variety of clinical lesions. To understand the molecular differences among these viruses that result in lesions with distinct pathologies, we have begun a MS-based proteomic analysis of HPV-host cellular protein interactions and have created the plasmid and cell line libraries required for these studies. To validate our system, we have characterized the host cellular proteins that bind to the E7 proteins expressed from 17 different HPV types. These studies reveal a number of interactions, some of which are conserved across HPV types and others that are unique to a single HPV species or HPV genus. Binding of E7 to UBR4/p600 is conserved across all virus types, whereas the cellular protein EN

SUBMITTER: White EA 

PROVIDER: S-EPMC3277141 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature