Enzyme-linked immunosorbent assay-based method to quantify the association of small molecules with aggregated amyloid peptides.

ABSTRACT: This paper describes a simple enzyme linked immunosorbent assay (ELISA) protocol for quantifying the binding of small molecules to aggregated β-amyloid (Aβ) peptides. Amyloid-targeting small molecules have attracted wide interest as potential agents for the treatment or diagnosis of neurodegenerative disorders such as Alzheimer's disease. The lack of general methods to evaluate small molecule-amyloid binding interactions, however, has significantly limited the number of amyloid-targeting molecules that have been studied to date. Here, we demonstrate a general method to quantify small molecule-amyloid binding interactions via a modified quantitative ELISA protocol. A key feature of this protocol is the treatment of commercial ELISA plates with an air plasma to help maintain the desired β-sh

SUBMITTER: Capule CC 

PROVIDER: S-EPMC3277653 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature