Mad2 and Mad3 cooperate to arrest budding yeast in mitosis.

ABSTRACT:

Background

The spindle checkpoint ensures accurate chromosome transmission by delaying chromosome segregation until all chromosomes are correctly aligned on the mitotic spindle. The checkpoint is activated by kinetochores that are not attached to microtubules or are attached but not under tension and arrests cells at metaphase by inhibiting the anaphase-promoting complex (APC) and its coactivator Cdc20. Despite numerous studies, we still do not understand how the checkpoint proteins coordinate with each other to inhibit APC(Cdc20) activity.

Results

To ask how the checkpoint components induce metaphase arrest, we constructed fusions of checkpoint proteins and expressed them in the budding yeast Saccharomyces cerevisiae to mimic possible protein interactions during checkpoint

SUBMITTER: Lau DT 

PROVIDER: S-EPMC3277655 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature