Opposing effects of Runx2 and estradiol on breast cancer cell proliferation: in vitro identification of reciprocally regulated gene signature related to clinical letrozole responsiveness.

ABSTRACT:

Purpose

To assess the clinical significance of the interaction between estrogen and Runx2 signaling, previously shown in vitro.

Experimental design

MCF7/Rx2(dox) breast cancer cells were treated with estradiol and/or doxycycline to induce Runx2, and global gene expression was profiled to define genes regulated by estradiol, Runx2, or both. Anchorage-independent growth was assessed by soft-agar colony formation assays. Expression of gene sets defined using the MCF7/Rx2(dox) system was analyzed in pre- and on-treatment biopsies from hormone receptor-positive patients undergoing neoadjuvant letrozole treatment in two independent studies, and short-term changes in gene expression were correlated with tumor size reduction or Ki67 index at surgery.

Results

Reflecting its on

SUBMITTER: Chimge NO 

PROVIDER: S-EPMC3277803 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature