Chemically programmed antibodies targeting multiple alpha(v) integrins and their effects on tumor-related functions in vitro.

ABSTRACT: Integrins αvβ3 and αvβ6 are highly expressed on tumor cells and/or by the tumor vasculature of many human cancers, and represent promising targets for anticancer therapy. Novel chemically programmed antibodies (cpAbs) targeting these integrins were prepared using the catalytic aldolase Antibody (Ab) programming strategy. The effects of the cpAbs on cellular functions related to tumor progression were examined in vitro using tumor cell lines and their cognate integrin ligands, fibronectin and osteopontin. The inhibitory functions of the conjugates and their specificity were examined based on interference with cell-cell and cell-ligand interactions related to tumor progression. Cell binding analyses of the anti-integrin cpAbs revealed high affinity for tumor cells that overexpressed αvβ3 and

SUBMITTER: Goswami RK 

PROVIDER: S-EPMC3277862 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature