Prostaglandin receptors EP and FP are regulated by estradiol and progesterone in the uterus of ovariectomized rats.

ABSTRACT:

Background

Prostaglandins are important for female reproduction. Prostaglandin-E2 acts via four different receptor subtypes, EP1, EP2, EP3 and EP4 whereas prostaglandin-F2alpha acts through FP. The functions of prostaglandins depend on the expression of their receptors in different uterine cell types. Our aim was to investigate the expression of EPs and FP in rat uterus and to identify the regulation by estradiol, progesterone and estrogen receptor (ER) selective agonists.

Methods

We performed four different rat experiments involving treatments with estradiol, progesterone and ER agonists. Real-time PCR and immunohistochemistry were employed to evaluate receptor expression.

Results

Our results showed that all mRNAs and proteins of EPs and FP are expressed in the rat u

SUBMITTER: Blesson CS 

PROVIDER: S-EPMC3278370 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature