Development of the bi-partite Gal4-UAS system in the African malaria mosquito, Anopheles gambiae.

ABSTRACT: Functional genetic analysis in Anopheles gambiae would be greatly improved by the development of a binary expression system, which would allow the more rapid and flexible characterisation of genes influencing disease transmission, including those involved in insecticide resistance, parasite interaction, host and mate seeking behaviour. The Gal4-UAS system, widely used in Drosophila melanogaster functional genetics, has been significantly modified to achieve robust application in several different species. Towards this end, previous work generated a series of modified Gal4 constructs that were up to 20 fold more active than the native gene in An. gambiae cells. To examine the Gal4-UAS system in vivo, transgenic An. gambiae driver lines carrying a modified Gal4 gene under the control of the

SUBMITTER: Lynd A 

PROVIDER: S-EPMC3278442 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature