Distinct and complementary information provided by use of tissue and DNA microarrays in the study of breast tumor markers.

ABSTRACT: Emerging high-throughput screening technologies are rapidly providing opportunities to identify new diagnostic and prognostic markers and new therapeutic targets in human cancer. Currently, cDNA arrays allow the quantitative measurement of thousands of mRNA expression levels simultaneously. Validation of this tool in hospital settings can be done on large series of archival paraffin-embedded tumor samples using the new technique of tissue microarray. On a series of 55 clinically and pathologically homogeneous breast tumors, we compared for 15 molecules with a proven or suspected role in breast cancer, the mRNA expression levels measured by cDNA array analysis with protein expression levels obtained using tumor tissue microarrays. The validity of cDNA array and tissue microarray data were f

SUBMITTER: Ginestier C 

PROVIDER: S-EPMC3278769 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature