Investigation and identification of protein γ-glutamyl carboxylation sites.

ABSTRACT:

Background

Carboxylation is a modification of glutamate (Glu) residues which occurs post-translation that is catalyzed by γ-glutamyl carboxylase in the lumen of the endoplasmic reticulum. Vitamin K is a critical co-factor in the post-translational conversion of Glu residues to γ-carboxyglutamate (Gla) residues. It has been shown that the process of carboxylation is involved in the blood clotting cascade, bone growth, and extraosseous calcification. However, studies in this field have been limited by the difficulty of experimentally studying substrate site specificity in γ-glutamyl carboxylation. In silico investigations have the potential for characterizing carboxylated sites before experiments are carried out.

Results

Because of the importance of γ-glutamyl carboxylation in

SUBMITTER: Lee TY 

PROVIDER: S-EPMC3278826 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature