Identification of c-Myb Target Genes in K562 Cells Reveals a Role for c-Myb as a Master Regulator.

ABSTRACT: The c-Myb transcription factor is an important regulator of hematopoietic cell development. c-Myb is expressed in immature hematopoietic cells and plays a direct role in lineage fate selection, cell cycle progression, and differentiation of myeloid as well as B- and T-lymphoid progenitor cells. As a DNA-binding transcription factor, c-Myb regulates specific gene programs through activation of target genes. Still, our understanding of these programs is incomplete. Here, we report a set of novel c-Myb target genes, identified using a combined approach: specific c-Myb knockdown by 2 different siRNAs and subsequent global expression profiling, combined with the confirmation of direct binding of c-Myb to the target promoters by ChIP assays. The combination of these 2 approaches, as well as addi

SUBMITTER: Lorenzo PI 

PROVIDER: S-EPMC3278898 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature