In vitro murine leukemia retroviral integration and structure fluctuation of target DNA.

ABSTRACT: Integration of the retroviral genome into host DNA is a critical step in the life cycle of a retrovirus. Although assays for in vitro integration have been developed, the actual DNA sequences targeted by murine leukemia retrovirus (MLV) during in vitro reproduction are unknown. While previous studies used artificial target sequences, we developed an assay using target DNA sequences from common MLV integration sites in Stat5a and c-myc in the genome of murine lymphomas and successfully integrated MLV into the target DNA in vitro. We calculated the free energy change during folding of the target sequence DNA and found a close correlation between the calculated free energy change and the number of integrations. Indeed, the integrations closely correlated with fluctuation of the structure of t

SUBMITTER: Tsuruyama T 

PROVIDER: S-EPMC3279379 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature