Identification of a serum-induced transcriptional signature associated with type 1 diabetes in the BioBreeding rat.

ABSTRACT:

Objective

Inflammatory mediators associated with type 1 diabetes are dilute and difficult to measure in the periphery, necessitating development of more sensitive and informative biomarkers for studying diabetogenic mechanisms, assessing preonset risk, and monitoring therapeutic interventions.

Research design and methods

We previously utilized a novel bioassay in which human type 1 diabetes sera were used to induce a disease-specific transcriptional signature in unrelated, healthy peripheral blood mononuclear cells (PBMCs). Here, we apply this strategy to investigate the inflammatory state associated with type 1 diabetes in biobreeding (BB) rats.

Results

Consistent with their common susceptibility, sera of both spontaneously diabetic BB DRlyp/lyp and diabetes inducibl

SUBMITTER: Kaldunski M 

PROVIDER: S-EPMC3279523 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature