Mesenchymal stem cells enhance allogeneic islet engraftment in nonhuman primates.

ABSTRACT:

Objective

To test the graft-promoting effects of mesenchymal stem cells (MSCs) in a cynomolgus monkey model of islet/bone marrow transplantation.

Research design and methods

Cynomolgus MSCs were obtained from iliac crest aspirate and characterized through passage 11 for phenotype, gene expression, differentiation potential, and karyotype. Allogeneic donor MSCs were cotransplanted intraportally with islets on postoperative day (POD) 0 and intravenously with donor marrow on PODs 5 and 11. Recipients were followed for stabilization of blood glucose levels, reduction of exogenous insulin requirement (EIR), C-peptide levels, changes in peripheral blood T regulatory cells, and chimerism. Destabilization of glycemia and increases in EIR were used as signs of rejection; additional i

SUBMITTER: Berman DM 

PROVIDER: S-EPMC3279532 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature