Genetic link between obesity and MMP14-dependent adipogenic collagen turnover.

ABSTRACT:

Objective

In white adipose tissue, adipocytes and adipocyte precursor cells are enmeshed in a dense network of type I collagen fibrils. The fate of this pericellular collagenous web in diet-induced obesity, however, is unknown. This study seeks to identify the genetic underpinnings of proteolytic collagen turnover and their association with obesity progression in mice and humans.

Research design and methods

The hydrolysis and degradation of type I collagen at early stages of high-fat diet feeding was assessed in wild-type or MMP14 (MT1-MMP)-haploinsufficient mice using immunofluorescent staining and scanning electron microscopy. The impact of MMP14-dependent collagenolysis on adipose tissue function was interrogated by transcriptome profiling with cDNA microarrays. Genetic a

SUBMITTER: Chun TH 

PROVIDER: S-EPMC3279534 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature