The medial amygdalar nucleus: a novel glucose-sensing region that modulates the counterregulatory response to hypoglycemia.

ABSTRACT:

Objective

To determine whether the medial amygdalar nucleus (MAN) represents a novel brain glucose-sensing region involved in the detection of hypoglycemia and generation of a counterregulatory hormone response.

Research design and methods

Fura-2 calcium imaging was used to assess glucose responsivity in neurons isolated from the MAN and single-cell real-time reverse transcription PCR used to examine gene expression within glucose-responsive neurons. In vivo studies with local MAN perfusion of the glucoprivic agent, 2-deoxyglucose (2-DG), under normal and hypoglycemic conditions and also after MAN lesioning with ibotenic acid, were used to examine the functional role of MAN glucose sensors. In addition, retrograde neuronal tracer studies were used to examine reciprocal pathw

SUBMITTER: Zhou L 

PROVIDER: S-EPMC3279559 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature