Direct detection and serogroup characterization of Neisseria meningitidis from outbreak of meningococcal meningitis in Delhi.

ABSTRACT:

Background and objectives

Rapid clinical manifestation/progression of the meningococcal meningitis and lacunae in conventional bacteriological test often encourages indiscriminate use of antibiotics much before the etiology is established. Accordingly this study was planned to evaluate ctrA PCR for rapid molecular detection. In addition, multiplex PCR and sequencing was done for serogroup prediction to provide essential epidemiological and laboratory evidence for decision makers of health department of the country for choosing appropriate vaccine and phylogenetic analysis to establish its lineage.

Materials and methods

73 CSF samples, collected from equal number of suspected cases, were investigated by both bacteriological (microscopy, culture, LA and drug sensitivity testin

SUBMITTER: Negi S 

PROVIDER: S-EPMC3279770 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature