Comparison of Microscopy and PCR-RFLP for detection of Anaplasma marginale in carrier cattle.

ABSTRACT:

Background and objectives

In Iran, anaplasmosis is normally diagnosed with traditional Giemsa staining method. This is not applicable for identification of the carrier animals. The aim of this study was to compare the detection of Anaplasma marginale in two different numbers of microscopic fields (50 and 100) using conventional Giemsa staining method compared with the PCR-RFLP technique.

Materials and methods

In this study, examinations were performed on 150 blood samples from cattle without clinical signs. Sensitivity and specificity of two microscopic fields (50 and 100 fields) were compared with A. marginale specific PCR-RFLP. The degree of agreement between PCR-RFLP and the two microscopic tests was determined by Kappa (κ) values with 95% confidence intervals.

Results

SUBMITTER: Noaman V 

PROVIDER: S-EPMC3279773 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature