The influence of riboflavin and nicotinic acid on Shigella sonnei colony conversion.

ABSTRACT:

Background and objectives

Shigella, causative of bacillary dysentery, has two colony forms. The loss of large virulence plasmid from virulent Shigella sonnei form I, during cell storage and subculturing, lead to avirulent form II. Environmental factors, e.g. culture media composition, could affect the conversion of the bacterial forms.

Materials and methods

In this study, some components, i.e., B-complex vitamins, nicotinic acid and riboflavin, were added to the bacterial culture medium and their influence on colony conversion were examined.

Results

The findings revealed that colony conversion is temperature independent and growth on the SS agar did not stabilize the bacterium in form I. Also, the findings showed that colonies on the minimal media supplemented with ni

SUBMITTER: Deldar A 

PROVIDER: S-EPMC3279797 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature