Cell surface proteins in S. pneumoniae, S. mitis and S. oralis.

ABSTRACT:

Background and objectives

Streptococcus pneumoniae, a major human pathogen, is closely related to the commensal species S. mitis and S. oralis. S. pneumoniae surface proteins are implicated in virulence and host interaction of this species, but many of them have recently been detected in S. mitis B6 in silico. We tested for the presence of such genes usinga set of eight S. mitis and eleven S. oralis strains from different geographic locations.

Materials and methods

An oligonucleotide microarray was designed based on the genomes of S. pneumoniae R6 and TIGR4 as well as S. mitis B6 to include 63 cell surface proteins. The S. pneumoniae genes encoding neuraminidases, hyaluronidase and pneumolysin were also included. In addition to comparative genomic hybridization experiments,

SUBMITTER: Madhour A 

PROVIDER: S-EPMC3279804 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature