Isolation and identification of a novel, lipase-producing bacterium, Pseudomnas aeruginosa KM110.

ABSTRACT:

Background and objectives

Lipases are particularly important due to the fact that they specifically hydrolyze acyl glycerol, oils and greases, which is of great interest for different industrial applications.

Materials and methods

In this study, several lipase-producing bacteria were isolated from wastewater of an oil processing plant. The strain possessing the highest lipase activity was identified both biochemically and sequencing of 16S rRNA gene. Then we increase lipase activity by improving conditions of production medium. Also, lipase from this strain was preliminarily characterized for use in industrial application.

Results

The 16S rRNA sequensing revealed it as a new strain of Pseudomonas aeruginosa and the type strain was KM110. An overall 3-fold enhanced lip

SUBMITTER: Mobarak-Qamsari E 

PROVIDER: S-EPMC3279805 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature