Evaluation of pathogenic serovars of Leptospira interrogans in dairy cattle herds of Shahrekord by PCR.

ABSTRACT:

Background and objectives

Leptospirosis is an important zoonotic disease caused by Leptospira interrogans. Leptospirosis leads to economical losses in dairy farm industry. The objective of this study was to evaluate the pathogenic serovars of Leptospira interrogans in dairy cattle herds of Shahrekord by PCR.

Materials and methods

Two hundred samples (100 urine and 100 blood) were collected from 100 cows randomly and delivered to the laboratory. Samples were stored at -20 °C. DNA was extracted and purified from the plasma and urine samples and concentrated on diatoms in the presence of guanidine thiocyanate (GuSCN). PCR products were detected and identified as Leptospira by ilumination of the expected size of DNA bands after staining of the agarose gel with ethidium bromide g

SUBMITTER: Jafari Dehkordi A 

PROVIDER: S-EPMC3279815 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature