First report of vannellidae amoebae (vannella spp.) isolated from biofilm source.

ABSTRACT:

Background

Members of the Vannellidae family are free-living amoebae (FLA) distributed mainly in water and soil sources. The present study reports the first isolation of this genus in the biofilm source from hospital environment in Tehran, Iran.

Methods

Biofilm samples were collected from hospital environment. Cultivation was performed in non-nutrient agar covered with a heat-killed Escherichia coli. Cloning of the suspected amoebae was done. PCR amplification and Homology analysis using the Basic Local Alignment Search Tool (BLASTn) was performed to search for the most similar reference sequences.

Results

Microscopic examination showed numerous fan-shaped amoebae and peculiar cysts different to the usual shape of typical FLA. Sequence analysis of the PCR- product rev

SUBMITTER: Lasjerdi Z 

PROVIDER: S-EPMC3279898 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature