Peripheral blood gene expression: it all boils down to the RNA collection tubes.

ABSTRACT:

Background

Gene expression profiling from peripheral blood is a valuable tool for biomarker discovery in clinical studies. Different whole blood RNA collection and processing methods are highly variable and might confound comparisons of results across studies. The main aim of the study was to compare genome-wide gene expression profiles obtained from the two widely used commercially available whole blood RNA collection systems - PAXgene™ and Tempus™ tubes. Comparisons of present call rates, variances, correlations and influence of globin reduction across the two collection systems was performed using in vivo glucocorticoid stimulation in 24 peripheral blood samples from three individuals.

Results

RNA quality, yield and numbers of detected transcripts from the two RNA collect

SUBMITTER: Menke A 

PROVIDER: S-EPMC3280191 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature