Use of non-amplified RNA samples for microarray analysis of gene expression.

ABSTRACT: Demand for high quality gene expression data has driven the development of revolutionary microarray technologies. The quality of the data is affected by the performance of the microarray platform as well as how the nucleic acid targets are prepared. The most common method for target nucleic acid preparation includes in vitro transcription amplification of the sample RNA. Although this method requires a small amount of starting material and is reported to have high reproducibility, there are also technical disadvantages such as amplification bias and the long, laborious protocol. Using RNA derived from human brain, breast and colon, we demonstrate that a non-amplification method, which was previously shown to be inferior, could be transformed to a highly quantitative method with a dynamic r

SUBMITTER: Sudo H 

PROVIDER: S-EPMC3280296 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature