Identification of differential gene expressions in colorectal cancer and polyp by cDNA microarray.

ABSTRACT:

Aim

To screen the differential expressed genes in colorectal cancer and polyp tissue samples.

Methods

Tissue specimens containing 16 cases of colorectal adenocarcinoma and colorectal polyp vs normal mucosae were collected and subjected to cDNA microarray and bioinformatical analyses. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to confirm some of the cDNA microarray data.

Results

The experimental data showed that eight genes were differentially expressed, most of which were upregulated in adenomatous polyp lesions. Forty-six genes expressions were altered in colorectal cancers, of which 29 were upregulated and 17 downregulated, as compared to the normal mucosae. In addition, 18 genes were similarly altered in both adenomatous polyps

SUBMITTER: Dai YC 

PROVIDER: S-EPMC3280404 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature