Primary human monocyte differentiation regulated by Nigella sativa pressed oil.

ABSTRACT:

Background

Oxidized low density lipoprotein plays an important role in development of foam cells in atherosclerosis. The study was focused on regulation of primary human monocyte growth and CD11b expression in presence of Nigella sativa oil.

Methods

Primary human monocytes were isolated from whole blood and grown at 37°C and 5% CO₂ saturation for five days prior to treatment with Nigella sativa oil. The cells were plated and washed before treatment with ox-LDL (10 μg/ml) as positive control and combined treatment of ox-LDL (10 μg/ml) and (140 ng/ml) Nigella sativa oil. The growth progression was monitored every 24 hours for 3 days.

Results

Macrophages showed reduced growth in comparison to monocytes 24 hours after treatment with Nigella sativa oil. The mean cell diame

SUBMITTER: Mat MC 

PROVIDER: S-EPMC3280944 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature