Co-culture with Schwann cells is an effective way for adipose-derived stem cells neural transdifferentiation.

ABSTRACT:

Introduction

Adipose-derived stem cells (ADSCs) could accomplish neural transdifferentiation with the presence of certain growth factors in vitro. It has been proved that bone marrow stromal cells (BMSCs) can realize neural transdifferentiation only by being co-cultured with Schwann cells (SCs), and in our former studies we have confirmed that ADSCs could do so too. This paper aims to investigate whether the neural induction efficiency of co-culture is as high as that of other strategies using chemicals or chemicals combined with some growth factors.

Material and methods

We isolated and multiplied ADSCs from adult Sprague-Dawley rats, and SCs from sciatic nerves of 1-to-2-day-old Sprague-Dawley rat pups, then induced ADSCs neural transdifferentiation through 2% dimethyl sulp

SUBMITTER: Liao D 

PROVIDER: S-EPMC3281332 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature