Metallothionein (MT) -I and MT-II expression are induced and cause zinc sequestration in the liver after brain injury.

ABSTRACT:

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Experiments with transgenic over-expressing, and null mutant mice have determined that metallothionein-I and -II (MT-I/II) are protective after brain injury. MT-I/II is primarily a zinc-binding protein and it is not known how it provides neuroprotection to the injured brain or where MT-I/II acts to have its effects. MT-I/II is often expressed in the liver under stressful conditions but to date, measurement of MT-I/II expression after brain injury has focused primarily on the injured brain itself. In the present study we measured MT-I/II expression in the liver of mice after cryolesion brain injury by quantitative reverse-transcriptase PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) with the UC1MT antibody. Displacement curves constructed using MT-I/II knockout

SUBMITTER: Pankhurst MW 

PROVIDER: S-EPMC3281953 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature