Accelerated identification of proteins by mass spectrometry by employing covalent pre-gel staining with Uniblue A.

ABSTRACT:

Background

The identification of proteins by mass spectrometry is a standard method in biopharmaceutical quality control and biochemical research. Prior to identification by mass spectrometry, proteins are usually pre-separated by electrophoresis. However, current protein staining and de-staining protocols are tedious and time consuming, and therefore prolong the sample preparation time for mass spectrometry.

Methodology and principal findings

We developed a 1-minute covalent pre-gel staining protocol for proteins, which does not require de-staining before the mass spectrometry analysis. We investigated the electrophoretic properties of derivatized proteins and peptides and studied their behavior in mass spectrometry. Further, we elucidated the preferred reaction of proteins

SUBMITTER: Mata-Gomez MA 

PROVIDER: S-EPMC3281962 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature