The nestin-expressing and non-expressing neurons in rat basal forebrain display different electrophysiological properties and project to hippocampus.

ABSTRACT:

Background

Nestin-immunoreactive (nestin-ir) neurons have been identified in the medial septal/diagonal band complex (MS/DBB) of adult rat and human, but the significance of nestin expression in functional neurons is not clear. This study investigated electrophysiological properties and neurochemical phenotypes of nestin-expressing (nestin+) neurons using whole-cell recording combined with single-cell RT-PCR to explore the significance of nestin expression in functional MS/DBB neurons. The retrograde labelling and immunofluorescence were used to investigate the nestin+ neuron related circuit in the septo-hippocampal pathway.

Results

The results of single-cell RT-PCR showed that 87.5% (35/40) of nestin+ cells expressed choline acetyltransferase mRNA (ChAT+), only 44.3% (35/79

SUBMITTER: Zhu J 

PROVIDER: S-EPMC3282673 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature