Sestrin2 modulates AMPK subunit expression and its response to ionizing radiation in breast cancer cells.

ABSTRACT:

Background

The sestrin family of stress-responsive genes (SESN1-3) are suggested to be involved in regulation of metabolism and aging through modulation of the AMPK-mTOR pathway. AMP-activated protein kinase (AMPK) is an effector of the tumour suppressor LKB1, which regulates energy homeostasis, cell polarity, and the cell cycle. SESN1/2 can interact directly with AMPK in response to stress to maintain genomic integrity and suppress tumorigenesis. Ionizing radiation (IR), a widely used cancer therapy, is known to increase sestrin expression, and acutely activate AMPK. However, the regulation of AMPK expression by sestrins in response to IR has not been studied in depth.

Methods and findings

Through immunoprecipitation we observed that SESN2 directly interacted with the AMPKα

SUBMITTER: Sanli T 

PROVIDER: S-EPMC3282792 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature