Sequencing genes in silico using single nucleotide polymorphisms.

ABSTRACT:

Background

The advent of high throughput sequencing technology has enabled the 1000 Genomes Project Pilot 3 to generate complete sequence data for more than 906 genes and 8,140 exons representing 697 subjects. The 1000 Genomes database provides a critical opportunity for further interpreting disease associations with single nucleotide polymorphisms (SNPs) discovered from genetic association studies. Currently, direct sequencing of candidate genes or regions on a large number of subjects remains both cost- and time-prohibitive.

Results

To accelerate the translation from discovery to functional studies, we propose an in silico gene sequencing method (ISS), which predicts phased sequences of intragenic regions, using SNPs. The key underlying idea of our method is to infer diplo

SUBMITTER: Zhang XC 

PROVIDER: S-EPMC3283449 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature