Cloning and expression of an endo-1,4-β-xylanase from the coffee berry borer, Hypothenemus hampei.

ABSTRACT:

Background

The coffee berry borer, Hypothenemus hampei, reproduces and feeds exclusively on the mature endosperm of the coffee seed, which has a cell wall composed mainly of a heterogeneous mixture of hemicellulose polysaccharides, including arabinoxylans. Xylanases are digestive enzymes responsible for the degradation of xylan based polymers, hydrolyzing them into smaller molecules that are easier to assimilate by insects. We report the cloning, expression and enzymatic characterization of a xylanase gene that was identified in the digestive tract of the coffee berry borer.

Methods

The complete DNA sequence encoding a H. hampei xylanase (HhXyl) was obtained using a genome walking technique in a cDNA library derived from the borer digestive tract. The XIP-I gene was amplifie

SUBMITTER: Padilla-Hurtado B 

PROVIDER: S-EPMC3283504 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature