Novel immune-modulator identified by a rapid, functional screen of the parapoxvirus ovis (Orf virus) genome.

ABSTRACT:

Background

The success of new sequencing technologies and informatic methods for identifying genes has made establishing gene product function a critical rate limiting step in progressing the molecular sciences. We present a method to functionally mine genomes for useful activities in vivo, using an unusual property of a member of the poxvirus family to demonstrate this screening approach.

Results

The genome of Parapoxvirus ovis (Orf virus) was sequenced, annotated, and then used to PCR-amplify its open-reading-frames. Employing a cloning-independent protocol, a viral expression-library was rapidly built and arrayed into sub-library pools. These were directly delivered into mice as expressible cassettes and assayed for an immune-modulating activity associated with parapoxvir

SUBMITTER: McGuire MJ 

PROVIDER: S-EPMC3283511 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature