System-wide immunohistochemical analysis of protein co-localization.

ABSTRACT:

Background

The analysis of co-localized protein expression in a tissue section is often conducted with immunofluorescence histochemical staining which is typically visualized in localized regions. On the other hand, chromogenic immunohistochemical staining, in general, is not suitable for the detection of protein co-localization. Here, we developed a new protocol, based on chromogenic immunohistochemical stain, for system-wide detection of protein co-localization and differential expression.

Methodology/principal findings

In combination with a removable chromogenic stain, an efficient antibody stripping method was developed to enable sequential immunostaining with different primary antibodies regardless of antibody's host species. Sections were scanned after each staining, a

SUBMITTER: Kim M 

PROVIDER: S-EPMC3283725 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature