Excitation spectra and brightness optimization of two-photon excited probes.

ABSTRACT: Two-photon probe excitation data are commonly presented as absorption cross section or molecular brightness (the detected fluorescence rate per molecule). We report two-photon molecular brightness spectra for a diverse set of organic and genetically encoded probes with an automated spectroscopic system based on fluorescence correlation spectroscopy. The two-photon action cross section can be extracted from molecular brightness measurements at low excitation intensities, while peak molecular brightness (the maximum molecular brightness with increasing excitation intensity) is measured at higher intensities at which probe photophysical effects become significant. The spectral shape of these two parameters was similar across all dye families tested. Peak molecular brightness spectra, which ca

SUBMITTER: Mutze J 

PROVIDER: S-EPMC3283774 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature