Accelerating the inbreeding of multi-parental recombinant inbred lines generated by sibling matings.

ABSTRACT: Inbred model organisms are powerful tools for genetic studies because they provide reproducible genomes for use in mapping and genetic manipulation. Generating inbred lines via sibling matings, however, is a costly undertaking that requires many successive generations of breeding, during which time many lines fail. We evaluated several approaches for accelerating inbreeding, including the systematic use of back-crosses and marker-assisted breeder selection, which we contrasted with randomized sib-matings. Using simulations, we explored several alternative breeder-selection methods and monitored the gain and loss of genetic diversity, measured by the number of recombination-induced founder intervals, as a function of generation. For each approach we simulated 100,000 independent lines to es

SUBMITTER: Welsh CE 

PROVIDER: S-EPMC3284326 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature